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propidium iodide pi  (Dojindo Labs)
95
Dojindo Labs propidium iodide pi
Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells <t>(Propidium</t> iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.
Propidium Iodide Pi, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pi staining solution  (Thermo Fisher)
99
Thermo Fisher pi staining solution
Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells <t>(Propidium</t> iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.
Pi Staining Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pi staining solution - by Bioz Stars, 2026-07
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propidium iodide pi staining solution  (Servicebio Inc)
86
Servicebio Inc propidium iodide pi staining solution
Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells <t>(Propidium</t> iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.
Propidium Iodide Pi Staining Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/propidium+iodide+%28pi%29+solution/pmc13263685-246-7-12?v=Servicebio+Inc
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propidium iodide pi staining solution  (Yeasen Biotechnology)
86
Yeasen Biotechnology propidium iodide pi staining solution
Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells <t>(Propidium</t> iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.
Propidium Iodide Pi Staining Solution, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/propidium+iodide+%28pi%29+solution/pmc13211853-105-10-15?v=Yeasen+Biotechnology
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propidium iodide pi  (Elabscience Biotechnology)
95
Elabscience Biotechnology propidium iodide pi
Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells <t>(Propidium</t> iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.
Propidium Iodide Pi, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/propidium+iodide+%28pi%29+solution/pmc13100712-267-50-66?v=Elabscience+Biotechnology
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propidium iodide pi - by Bioz Stars, 2026-07
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propidium iodide pi  (Miltenyi Biotec)
97
Miltenyi Biotec propidium iodide pi
Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells <t>(Propidium</t> iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.
Propidium Iodide Pi, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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propidium iodide pi solution  (Yeasen Biotechnology)
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Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells <t>(Propidium</t> iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.
Propidium Iodide Pi Solution, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/propidium+iodide+%28pi%29+solution/pmc13194797-406-8-12?v=Yeasen+Biotechnology
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propidium iodide pi solution - by Bioz Stars, 2026-07
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propidium iodide pi staining solution  (Thermo Fisher)
99
Thermo Fisher propidium iodide pi staining solution
Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells <t>(Propidium</t> iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.
Propidium Iodide Pi Staining Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/propidium+iodide+%28pi%29+solution/pmc13099090-219-9-22?v=Thermo+Fisher
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propidium iodide pi staining solution - by Bioz Stars, 2026-07
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propidium iodide pi solution  (Thermo Fisher)
99
Thermo Fisher propidium iodide pi solution
Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells <t>(Propidium</t> iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.
Propidium Iodide Pi Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/propidium+iodide+%28pi%29+solution/pmc13044302-264-12-20?v=Thermo+Fisher
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propidium iodide pi solution - by Bioz Stars, 2026-07
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Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells (Propidium iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: A fluffy nanofiber scaffold-based microenvironment enables a simplified, biology-centric scale-up strategy for mesenchymal stem/stromal cell culture

doi: 10.3389/fbioe.2026.1808384

Figure Lengend Snippet: Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells (Propidium iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.

Article Snippet: Sampled cells were rinsed with D-PBS(−) and stained with 1.0 μg/mL Calcein-AM (C326; DOJINDO) and 2.0 μg/mL Propidium iodide (PI) (P378; DOJINDO) in a multi-well plate for 15 min at 37 °C.

Techniques: Suspension, Comparison, Staining, Fluorescence, Cell Culture